Richard Henry, Sigma Aldrich, presents an online learning tutorial on "HPLC Column Fundamentals". This 25-minute session covers the basics of HPLC columns and the separation process. All content from this online seminar is applicable to HPLC columns independent of vendor.

What will you gain from the course?
An introduction to how solutes can interact with columns containing porous particles and stationary phases to result in separation. The efficiency, selectivity and retention terms and the resolution equation will be explained as well as how certain column particle characteristics can alter the terms and affect resolution.

What does it cover?
"HPLC Column Fundamentals" focuses on column and separation basics:
• resolution
• retention factor
• selectivity
• column efficiency
•column symmetry (USP tailing factor)
• porous silica particles

About the Instructor
Richard Henry received his B.S. degree in Chemistry from Juniata College in 1963 and Ph.D. in Analytical Chemistry from The Pennsylvania State University in 1966. After a postdoctoral year in separations at Purdue University with Professor L. B. Rogers, he joined DuPont in 1967 at the Experimental Station in Wilmington, DE and became one of the first employees of the DuPont Instrument Products Division in 1969. Dick worked closely with Dr. Jack Kirkland and others in the development of HPLC columns and packing materials while at DuPont. After about 10 years with Spectra-Physics and consulting on the west coast, he joined the Penn State University chemistry faculty in 1985 where he was Director of Analytical Laboratories and taught Instrumental Analysis to chemistry majors. In 1985, he also founded Keystone Scientific, Inc. where he developed HPLC columns and related separation technology. He retired from both Penn State University and Keystone Scientific in 2002, and remains active teaching short courses on separation technology and as a consultant. Dick has research interests in separation mechanisms and all applications of new LC column technology. He is also interested in new analytical techniques, especially the rapidly growing fields of UHPLC, chiral HPLC, LC-MS, and multidimensional separation methods. He served two terms as Chairman of the ACS Subdivision on Separations (1998-2002) and has also served for many years on its Executive Committee.

To access the e-Learning Module:
1. Complete the right-hand registration form below in full and click 'Register'.
2. Carefully read the 'Copyright Information' on the following page and if happy to proceed use the two buttons at the bottom of the page for access. Click 'View Seminar' to start the e-Learning Module and 'Download Handout' to download the related seminar materials. You will have unlimited access to this e-Learning Module for 1 month from registration.
 
For subsequent access session simply return to the login page and input your email address and password.